Profiling prefrontal cortex protein expression in rats exhibiting an incubation of copyright craving following short-access self-administration procedures

Profiling prefrontal cortex protein expression in rats exhibiting an incubation of copyright craving following short-access self-administration procedures

Blog Article

IntroductionIncubation of drug-craving refers to a time-dependent increase in drug cue-elicited craving that occurs during protracted withdrawal.Historically, rat models of incubated copyright craving employed extended-access (typically 6 h/day) intravenous drug self-administration (IV-SA) procedures, although incubated copyright craving is reported to occur following shorter-access IV-SA paradigms.The notoriously low-throughput of extended-access IV-SA prompted us to determine whether two different short-access IV-SA procedures akin to those in the literature result in qualitatively similar changes in glutamate receptor expression and the activation of downstream signaling molecules within prefrontal cortex LIQUID MAKEUP ALMOND BEIGE #1020 (PFC) subregions as those reported previously by our group under 6h-access conditions.MethodsFor this, adult, male Sprague-Dawley rats were trained to intravenously self-administer copyright for 2 h/day for 10 consecutive days (2-h model) or for 6 h on day 1 and 2 h/day for the remaining 9 days of training (Mixed model).

A sham control group was also included that did not self-administer copyright.ResultsOn withdrawal day 3 or 30, rats were subjected to a 2-h test of cue-reinforced responding in the absence of copyright and a time-dependent increase in drug-seeking Childs Table was observed under both IV-SA procedures.Immunoblotting of brain tissue collected immediately following the cue test session indicated elevated phospho-Akt1, phospho-CaMKII and Homer2a/b expression within the prelimbic subregion of the PFC of copyright-incubated rats.However, we failed to detect incubation-related changes in Group 1 metabotropic glutamate receptor or ionotropic glutamate receptor subunit expression in either subregion.

DiscussionThese results highlight further a role for Akt1-related signaling within the prelimbic cortex in driving incubated copyright craving, and provide novel evidence supporting a potential role also for CaMKII-dependent signaling through glutamate receptors in this behavioral phenomenon.